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. 2000 Mar 21;97(7):3148–3153. doi: 10.1073/pnas.070452397

Figure 6.

Figure 6

hRPB10α, but not hRPB2, mediates Sp1-dependent activation. All reactions contained the general transcription factors, USA fraction, and Pol II in the absence or presence of Sp1. (A) Effect on Sp1-dependent activated transcription by hRPB3 to hRPB11. For comparison, the activation in lane 2 (absence of added Pol II subunit) is 10-fold. (B) Effect Sp1-dependent transcription by hRPB2 peptides. (C) Titration, as in Fig. 2, of hRPB10α (lanes 7–9), but not hRPB2(315–606) (lanes 4–6), results in an incremental decrease in Sp1-dependent activated transcription.