Figure 2.

The activity of Gal3p deletion mutations. (A) Plasmids expressing the wild-type Gal3p protein or the deletion derivative indicated were transformed into a yeast strain in which the GAL3 gene had been disrupted. Each of the strains were then grown on either raffinose (Raf) or galactose (Gal) as the sole carbon source, and cell growth was monitored after 3 days of growth at 30°C. (B) The effect of various Gal3p deletion derivatives on the expression of GAL10. Northern blot analysis of GAL10 and 18S RNA expression was monitored daily after the cells were transferred to a galactose-containing medium. (C) Western blot analysis of Gal3p and the deletion derivatives. A yeast strain deleted for GAL1, GAL3, and GAL80 was transformed with the appropriate Gal3p expression vector and grown on galactose for 2 days. Western blot analysis was performed on whole cell extracts using either Gal3p polyclonal antibodies or 30C12, a mouse monoclonal antibody raised against Gal3p.