Figure 4.

The activity of Gal3p+SA in vitro. In vitro transcription and primer extension reactions were performed as described previously (9). All reactions contained 1.5 mM galactose and 1 mM ATP and, where indicated, 3 nM Gal4p (amino acids 1–93 fused to 768–881), 3 nM Gal80p and either Gal3p (75, 25, and 8 nM in lanes 4, 5, and 6, respectively), Gap3p+SA (75, 25, and 8 nM in lanes 7, 8, and 9, respectively), or Gal1p (3,000, 1,000, and 333 nM in lanes 10, 11, and 12 respectively).