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pH dependence of amide exchange for residues from P. furiosus rubredoxin at 28°C. Gly-9 (■), Gly-17 (●), Thr-27, (▴), and Glu-47 (⧫) illustrate residues distributed throughout the protein structure that give observable magnetization transfer cross peaks across a 1.5–2.0 pH unit span in the high pH range. EX2 behavior is observed up to pH 12.51. Best-fit lines of unit slope indicate proportionality between the exchange rate and [OH−].
