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Variation in fluorescent signals (noise) from 1000 randomly selected cells. (A) MEF cell line 33e1 (B) ES cell line R26CY1. The fluorescent units are relative values assigned by the sorter. Intensities for CFP have been normalized to correct for its relative faintness when compared to YFP intensity. Arrows at right angles indicate noise axes, extrinsic (E) and intrinsic (I).
