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. 2006 Oct 16;7:36. doi: 10.1186/1471-2199-7-36

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Copyright © 2006 Larson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Genotyping the ROSA26 locus in DNA isolated from monochromatic clones. At left are shown maps of the two alleles in parental cells. The YFP gene has an additional cleavage site for Pst1 restriction endonuclease. Lane 1, a clone lacking the YFP gene. Lane 2, a dichromatic clone, which retained both the genes, as expected. Lane 3, a clone lacking the CFP gene. Lanes 4 & 5, data from DNA of unsorted ES cell lines. Lane 6, PCR control containing no DNA in the reaction mix. Markers are 1 Kb DNA ladder (Invitrogen).