Figure 4.

Photomicrographs of the autoradiographic distribution of [¹²⁵I]-GR231118 binding sites in the rat brain. Adjacent coronal rat brain sections were incubated in the presence of 25 pM [¹²⁵I]-GR231118 (total binding, Total) and either 100 nM BIBO3304 (to block Y₁ sites), 100 nM hPP (to block Y₄ sites), 100 nM hPP (to block Y₄ sites) or 100 nM GR231118 (non-specific binding). Abbreviations: Acb, Accumbens; AD, anterodorsal thalamic nuclei; AM, anteromedial thalamic nuclei; AO, anterior olfactory nuclei; AP, area postrema; AV, anteroventral thalamic nuclei; BLA, basolateral amygdaloid nuclei; CA1, CA1 subfield of the hippocampus; CA2, CA2 subfield of the hippocampus; CA3, CA3 subfield of the hippocampus; Ce, cerebellum; CEA, Central amygdaloid nucleus; Cg, cingulate cortex; Cl, claustrum; CPu, caudate putamen; DG, dentate gurus; EPL, external plexiform layer of the olfactory bulb; Fr, frontal cortex; Hi, hippocampus; IC, inferior colliculus; ICj, Island of Calleja; IO, inferior olive; IP, interpeduncular nucleus; LA, lateroanterior hypothalamic nucleus; LD, laterodorsal thalamic nuclei; LH, lateral hypothalamic nucleus; LS, lateral septum; MD, mediodorsal thalamic nuclei, MG, medial geniculate nuclei, MM, mamilary nucleus; MPA, medial preoptic area; Par, parietal cortex; Pir, piriform cortex; PVN, paraventricular hypothalamic nucleus; Re, reuniens thalamic nucleus; Rh, Rhomboid thalamic nucleus; Sol, nucleus tractus solitarius; TT, tenia tecta; Tu, olfactory tubercle; VL, ventrolateral thalamic nuclei; VMH, ventromedial hypothalamic nucleus. Scale bar represents 10 mm.