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Nano-ESI-MS spectrum of LUVETs of (A) WALP16/DMPC (1:25) and (B) WALP16(+10)/DMPC (1:25). The [M+Na]+ ions of the DMPC monomer, dimer and trimer are most abundant; the isotope envelopes of the [M+Na]+ ions of the WALP16 and WALP16(+10) peptides are observed around m/z 1919 (A) and m/z 2559 (B); the [M+H]+ ions are around m/z 1897 (A) and m/z 2537 (B), respectively. For the recording of each spectrum, ≈200 nl of a vesicle suspension containing 20 μM peptide was used.
