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. 2000 Mar 21;97(7):3189–3194. doi: 10.1073/pnas.050444797

Figure 7.

Figure 7

Bar diagrams representing the average deuterium content of the partly deuterated A and Y′ fragment ions (26) (A) of WALP16 and (B) of WALP16(+10), as derived from nano-ESI-MS/MS measurements. The An fragment comprises the amide and side chain deuteriums of the N-terminal n amino acid residues; the Y′n fragment comprises the amide and side chain deuteriums of the C-terminal n amino acid residues plus the ethanolamine terminal group. The MS/MS spectra of the [M+Na]+ ions of WALP16 and WALP16(+10) were recorded when 12 of 22 and 20 of 32 hydrogens were exchanged, respectively. The deuterium content of the fragments as based on a simplified model (see text for explanation) is indicated by the black circles connected by a line (-●-). Data points for the fragments for which no bar is shown could not be obtained because of poor signal-to-noise ratio.