Fig. 6. Proliferation assay of Raftlin-deficient DT40 cells. (A) Growth curves of DT40 cells. Cells were suspended at 5 × 10⁴ cells per ml in the medium. They were cultured for the indicated time and the cell numbers were counted. (B) Cell cycle analysis. Various mutant DT40 cells were treated in hypotonic solution containing PI and subjected to DNA content analysis by FACScan.