Table 2.

Energy transfer measurements and calculated values

Samples*	Quench (E)	P values†	Separation, Å	% Monomers‡	Turnover, min−1§
Purified ATPase (10)	0.6260  ± 0.0315		49.6  ± 1.1	0	9,690  ± 340
Microsomes (19)	0.2822  ± 0.0287	<0.0001	63.1  ± 1.2	53.3  ± 4.6	9,473  ± 397
Microsomes, no phalloidin (10)	0.3378  ± 0.0338		60.4  ± 1.1	47.6  ± 5.4	9,476  ± 420
Microsomes + phalloidin (9)	0.2202  ± 0.0397	0.0183	66.7  ± 2.1	61.6  ± 6.3	9,470  ± 363

*Numbers in parentheses are number of FRET experiments. Values given in the table are the means ± standard error of the mean. 

^†The P values were determined by using an unpaired, one-tailed t-test. 

^‡ % Monomers were determined by assuming the purified ATPase was 100% dimer and dividing the energy transfer efficiency (E) of the microsomes by E (0.626) of the purified ATPase. 

^§Turnover values for purified ATPase are the mean ± SD from ref. 16. Turnover values for microsomes are the mean ± SD from three experiments ± phalloidin.