Figure 5.
RNA Gel Blot Analysis of Light-Regulated Genes in Response to White Light and Blue Light Irradiation.
(A) RNA samples were collected from wild-type Col seedlings (WT) and from two transgenic Arabidopsis lines deficient for AtPP7 (L4 and L5) in darkness (D), in response to 6 and 12 h of blue light irradiation (25 μmol·m−2·s−1) (B 6h and B12h), and in response to 6 and 12 h of white light irradiation (32 μmol·m−2·s−1) (WL 6h and WL 12h). The same membrane was probed with Rbcs, Cab, Chs, Sig5, CRY1, and CRY2. 18S rRNA was used as a loading control.
(B) RNA samples were collected from wild-type Landsberg erecta (WT) and hy4 seedlings in darkness and in response to 6 and 12 h of both white light and blue light irradiation (abbreviations as in [A]). The same membrane was probed with Chs, Sig5, CRY1, and CRY2. 18S rRNA was used as a loading control.