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. 2000 Mar 14;97(7):3224–3229. doi: 10.1073/pnas.050432997

Figure 2.

Figure 2

Levels of Sac3p affect cell cycle progression. (A) The growth of SAC3Δ cells transformed with vector alone (vec), wild-type SAC3 (Sac3), myc-tagged SAC3 (Sac3-myc), or GFP-tagged SAC3 (Sac3-GFP) is shown at 25°C (Left) and 37°C (Right). Growth was assessed by serial dilutions of logarithmically growing cultures. (B) Wild-type and SAC3Δ cells were grown to log phase in yeast extract/peptone/dextrose medium and then shifted to 37°C for 2 hr. Samples were stained with an antitubulin antibody to visualize microtubules and with 4′,6-diamidino-2-phenylindole to visualize DNA. (C) Wild-type cells were transformed with a galactose-inducible SAC3 plasmid (SAC3) or with the empty vector (vec). Cells were grown overnight in minimal medium containing raffinose. At time zero (0 h), expression of Sac3p was induced by the addition of galactose (2% final concentration). After a 3-hr induction (3 h), cells were prepared for indirect immunofluorescence and stained with antitubulin and 4′,6-diamidino-2-phenylindole.