FIG. 3.
Characterization of the interaction of the TPL-2 C terminus with p105. (A) Schematic diagram of recombinant p105 proteins. (B) Recombinant p105497-968 protein was digested with trypsin with or without TPL-2398-497 peptide for 60 min, resolved by 10% Bis-Tris gel electrophoresis, and Western blotted. Protein fragments were visualized by Coomassie brilliant blue staining. The mobilities of p105497-968 and the fragments corresponding to p105497-683 and p105534-683 are indicated. (C) Surface plasmon resonance analysis of the interaction of p105497-805 and p105540-801 protein with biotinylated TPL-2398-497 peptide immobilized on a streptavidin-coated sensor surface. An open arrow denotes the injection of biotinylated TPL-2 C-terminal peptide, closed arrow denotes the injection of indicated p105 protein. (D) Binding affinities of TPL-2398-497 peptide for the indicated recombinant p105 proteins, as determined by surface plasmon resonance.