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. 2003 Jul;23(14):5064–5077. doi: 10.1128/MCB.23.14.5064-5077.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 2. — SWI4 is required for viability of a stb1Δ deletion strain. (A) stb1Δ (BY1298), swi4Δ (BY184), and stb1Δ swi4Δ (BY1693) mutants harboring a high-copy SWI4-URA3 plasmid (pBA314) were grown on SD-URA medium or on SD medium containing 5-FOA. (B) stb1Δ (BY1298), swi4Δ (BY184) and stb1Δ swi4Δ (BY1694 and BY1695) mutants containing a SWI4-URA3 plasmid and harboring either a SWI4-LEU2 (pBA417), MET25pr-STB1-LEU2 (pBA1044), or vector (pRS425) plasmid were grown in the absence (SD-MET) or presence (SD-MET + 5-FOA) of 5-FOA.