FIG. 3.

Growth characteristics of the stb1Δ bck2Δ double mutant. (A) Slow-growth phenotype of a stb1Δ bck2Δ mutant strain. Tenfold serial dilutions were prepared from wild-type (BY263), stb1Δ (BY805), bck2Δ (BY1110), and stb1Δ bck2Δ (BY1106) cultures, plated onto YEPD medium, and incubated at 30°C. (B) Morphology of wild-type, stb1Δ, bck2Δ, and stb1Δ bck2Δ strains. Wild-type (BY263), stb1Δ (BY805), bck2Δ (BY1110), and stb1Δ bck2Δ (BY1106) strains were grown to mid-log phase in rich medium. The cells were viewed with Nomarski optics and photographed. (C) DNA content as measured by FACS analysis of samples shown in panel B. The positions of cells with G₁ or G₂ DNA contents are indicated by 1N or 2N, respectively. The percentages of budded and unbudded cells are indicated below the FACS profile.