FIG. 1.

MAbs against human LRP130. (A) Lysates from HeLa cells (lane 1) and from bacteria transformed with the C3 construct plasmid, encoding the 59-kDa C-terminal portion of LRP130, prior to or after induction with IPTG (lanes 2 and 3), and proteins from nmRNP complexes immunoprecipitated with a MAb against hnRNP A1 (lane 4) were resolved by SDS-PAGE and immunoblotted with the 9C9 MAb. Molecular mass markers, in kilodaltons, are indicated on the left. Ig h.c. and Ig l.c., antibody heavy and light chains, respectively. (B) Immunofluorescence localization of the LRP130 protein. HeLa cells grown on glass coverslips were fixed, permeabilized, and stained with MAb 9C9 (a and c) or with nonimmune myeloma immunoglobulins (d). (b) Phase microscopy image of the cells shown in panel a. All immunostaining images were obtained by using the same exposures.