FIG. 9.

The Hog1 double mutant induces high expression of target genes in the absence of osmotic stress. hog1Δ cells harboring plasmids with the indicated HOG1 alleles were grown to logarithmic phase in the presence of methionine. Then, cultures were split and methionine was removed from one half (time zero was after the split and methionine removal). Samples for RNA extraction were taken at the indicated time points. Northern blots (upper panels) were hybridized with ³²P-labeled probes corresponding to the Hog1 target genes GPD1, STL1, or the IPP1 gene that was used as a loading control. IPP1 was hybridized to the blots after stripping. Blots were quantified (by phosphorimager) and were normalized to IPP1 levels and plotted against time (lower panel).