FIG. 1.

Functional domains of Cdc15 and expression levels of various truncation constructs. (A) The N-terminal kinase domain (aa 1 to 267) is shown in purple; the self-association domain (aa 361 to 702) is shown in yellow; the SPB localization domain (aa 267 to 702) is shown in red, and the inhibitory domain (aa 751 to 974) is shown in green. (B) Anti-GFP Western blot analysis from strains grown in YEP plus 2% raffinose and induced with 2% galactose for 2.5 h. (*, cross-reacting bands; x, nonspecific mark on membrane). The following strains were used: A4683, A5575, A5576, A4692, A5578, A4678, A4690, A4685, A5464, A5466, A6456, and A5909.