FIG. 5.

Several GFP-CDC15 truncation constructs cause a telophase arrest upon overexpression. Strains were arrested for 3 h in G₁ with the α-factor pheromone (5 μg/ml) and induced with 2% galactose for the last hour. Cells were synchronously released into YEP plus 2% raffinose and 2% galactose, and samples were taken at indicated time points. (A) The key at right indicates strains A3932, A4441, and A4376 (top to bottom, respectively). Dashed lines represent metaphase spindles; solid lines represent anaphase/telophase spindles. (B) Growth conditions were as described for panel A, and the percentages of anaphase and telophase spindles are analyzed. The leftmost panel depicts class 1 Cdc15 constructs. The key shows strains A3932, A5574, A4695, A5576, A5051, A4691, and A4687 (top to bottom, respectively). Class 2 Cdc15 constructs are shown in the middle panel. The key shows strains A3932, A5580, A4680, A4677, and A6373 (top to bottom, respectively). The rightmost panel depicts class 3 Cdc15 constructs. The key shows strains A3932, A5464, A5909, A5466, A5914, A6456, and A4690 (top to bottom, respectively).