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. 2000 Mar 14;97(7):3242–3247. doi: 10.1073/pnas.060025597

Figure 1.

Figure 1

Effect of DA and Oxy on NKA activity, α-subunit phosphorylation and its abundance in clathrin vesicles, early and late endosomes. PCT cells were incubated with or without 1 μM DA (2.5 min at 23°C) in the presence or absence of 1 μM OXY. (A) NKA activity in microdissected PCT is the mean + SE of seven independent experiments performed in triplicate. (B) Effect of DA and Oxy on NKA α-subunit abundance in CCV, early endosomes, and late endosomes. A representative experiment (Upper) and the quantitative data of five experiments expressed as percent of control mean + SE (Lower) are shown. (C) NKA α-subunit phosphorylation. A representative experiment (Upper) indicating the state of α-subunit phosphorylation, and the quantitative data of five experiments expressed as percent of control mean + SE are shown in Lower.