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. 2003 Jun 15;31(12):3092–3100. doi: 10.1093/nar/gkg414

Figure 4.

Figure 4

Expression of Cc RNase in E.coli. Total extracts from equivalent amounts of E.coli harboring the expression plasmid PF were analyzed by SDS–PAGE and the gel was stained with Coomassie brilliant blue R250. Lane 1 shows the total cell extract before IPTG induction, whereas lanes 2 and 3 demostrate proteins obtained after IPTG induction at 2 and 4 h, respectively. Cc RNase is expressed as a fusion protein exhibiting a molecular mass of 45 kDa. Marker proteins of known molecular weight (94, 67, 43, 30, 20.1 and 14.4 kDa top to bottom) are presented in lane M.