Figure 5.

DIG association of Gas1p during biosynthetic transport. Cells [wild type (NY179) and sec18–1 (H891)] were preincubated at 24°C or 37°C for 5 min, pulse-labeled with [³⁵S]methionine for 5 min, and then chased for 0 and 45 min. The samples from the two time points were taken and subjected to TX100 extraction and density gradient centrifugation. Gas1p was immunoprecipitated from fractions 1 [insoluble (I)] and 4 [soluble (S)]; the mature (m) and precursor (p) forms are indicated.