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Electrophoretic mobility shift assay of triplex formation at pH 7.2. (A) dsDNA:TFO, 1:1; (B) dsDNA:TFO, 1:10. dsDNA of 5 µM and TFO of 5 or 50 µM were used. A solution of 50 mM Tris–HCl (pH 7.2 at 20°C) and 10 mM MgCl2 was used as the running buffer. MlnI reaction buffer described in Materials and Methods was used as the reaction buffer.
