. 2003 Jun 15;31(12):3267–3273. doi: 10.1093/nar/gkg416

Table 1. T_m values of triplexes containing ENA or 2′,4′-BNA/LNA at pH 6.6 and pH 7.2.

TFO	Sequence (5′→3′)	Modification	T_m (°C)
			pH 6.6	pH 7.2
DNA-1	TCTCTCTCCCTTTT	Natural	ND^a	ND^a
BNA-1	TCTCTCTCCCTTTT	BNA/LNA	41^a	ND^a
ENA-1	TCTCTCTCCCTTTT	ENA	52	ND
BNA-2	TCTCTCTCCCTTTT	BNA/LNA	41^a	ND^a
ENA-2	TCTCTCTCCCTTTT	ENA	39	ND
BNA-3	TCTCTCTCCCTTTT	BNA/LNA	ND^a	ND^a
ENA-3	TCTCTCTCCCTTTT	ENA	42	ND
T5mC	TCTCTCTCCCTTTT	Natural, 5-Me-C	28^a	ND
BNA-4	TCTCTCTCCCTTTT	BNA/LNA, 5-Me-C	54^a	37^a
ENA-4	TCTCTCTCCCTTTT	ENA, 5-Me-C	nd	ND
BNA-5	TCTCTCTCCCTTTT	BNA/LNA, 5-Me-C	55^a	33^a
ENA-5	TCTCTCTCCCTTTT	ENA, 5-Me-C	58	ND
BNA-6	TCTCTCTCCCTTTT	BNA/LNA, 5-Me-C	ND^a	ND^a
ENA-6	TCTCTCTCCCTTTT	ENA, 5-Me-C	57	41

Modified nucleotides are underlined. 5-Me-C, 5-methylcytidine analogs; ND, not detected; nd, not determined because the transition from the triplex to the duplex, although present, was not clear in the melting curve. The buffer was 10 mM phosphate buffer (pH 6.6 and 7.2) and 100 mM NaCl.

^aData from Obika et al. (5).

Table 1. Tm values of triplexes containing ENA or 2′,4′-BNA/LNA at pH 6.6 and pH 7.2.

Table 1. T_m values of triplexes containing ENA or 2′,4′-BNA/LNA at pH 6.6 and pH 7.2.