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. 2003 Jun 15;31(12):3194–3207. doi: 10.1093/nar/gkg419

Figure 3.

Figure 3

Multiple hCAR immunoreactive species are detected in whole-cell liver extracts. A western immunoblot of 200 µg/lane, whole-cell lysate from four different human livers is presented. Proteins were separated by electrophoresis on a 7.5% polyacrylamide gel and transferred to a PDVF membrane. The blot was probed with an antibody preparation generated in our laboratory (described in Materials and Methods). A number of immunoreactive bands (labeled 1–4) are apparent within the size range predicted for hCAR isoforms.