Figure 8.

All isoforms of hCAR interact specifically with the SRC-1 RID. (A) ³⁵S-Labeled hCAR protein isoforms expressed in rabbit reticulocyte lysate used for the GST pull-down experiments. Bands representing the [³⁵S]hCAR isoforms are indicated by arrows. (B) Bacterially expressed GST and GST–SRC-1-RID used in the GST pull-down experiments. Arrows indicate bands of the expected size. (C) GST pull-down experiments using immobilized GST–SRC-1-RID (even lanes) or GST (odd lanes) proteins and reticulocyte lysate expressed [³⁵S]hCAR protein isoforms. hCAR-REF interacted robustly and specifically with GST–SRC-1-RID (lane 2 versus lane 1). The other hCAR isoforms interacted specifically with GST–SRC-1-RID, but the interactions were weaker than that obtained with the reference isoform. Equal input c.p.m. levels were applied to each pull-down reaction.