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. 2000 Mar 28;97(7):3260–3265. doi: 10.1073/pnas.97.7.3260

Figure 3.

Figure 3

Northern blots of target genes. (A) RNA harvested from the indicated MYC-ER samples was used for Northern blots. Also depicted are Northern blot analysis results for RNA samples harvested from fibroblasts infected with a deletion mutant of the MYC-ER fusion protein incapable of transactivating MYC-responsive genes. Ethidium bromide-stained rRNA levels demonstrates similar loading in each lane. Fold inductions comparing MYC-ER infected cells with and without OHT treatment are 2.3 (Northern blot, experiment 1)/2.3 (microarray, experiment 1) and 2.2 (Northern blot, experiment 2)/2.1 (microarray, experiment 2) for FKBP52; 1.8/2.0 and 1.4/2.1 for PPIF; 4.1/3.6 for FABP5. (B) Northern blot analysis results for a fourth MYC-ER experiment are shown for eIF5A and cyclin D2. Fold inductions (MYC-ER+OHT/MYC-ER) are 1.8 (Northern blot)/2.3–3.0 (microarrays) for eIF5A and 3.5/2.2–5.7 for cyclin D2 (C). Northern blot analysis results for p21 showing repression of transcript levels after MYC-ER induction. Max was used as a loading control because its levels are not altered by MYC activation.