Figure 2.

Increased polyploidy in p19ARF-null fibroblasts after nocodazole treatment. Asynchronous cultures of p53^+/−, p53^−/−, p19ARF^−/−, and p16^−/−p19ARF^−/− MEFs were treated with or without 0.05 μg/ml nocodazole for 48 h, fixed, and stained with propidium iodide for FACS analysis. Values in the upper right corner of each plot represent the percentage of cells with >4N DNA content. Histogram plots of untreated or nocodazole-treated MEFs are shown in A (p53^+/−), B (p53^−/−), C (p19ARF^−/−), and D (p16^−/−p19ARF^−/−).