Fig. 4.

Transposition activity of GAL4 transposases. (A) A schematic representation of engineered chimeric transposases. A GAL4 DBD was fused in-frame to the N terminus of transposases, with a linker of 18–21 aa placed between GAL4 DBD and the transposases to facilitate proper domain folding. (B) The percentage of transposition activity relative to wild-type transposase in HEK293 cells. To compare the activity of each chimeric transposase to its wild-type counterpart, the wild-type transposition efficiency was normalized to 100%. Data are shown as mean values with SD (n = 6).