Table 2.

Analysis of transposon–chromosomal junction mediated by GAL4-piggyBac in CHO cells

Independent isolated clone	Donor plasmid TR	Chromosomal insertion site	Flanking chromosomal sequence
G8-2	5′-TGATTATCTTTCTAGGG	TTAA	GCTCGGGCCGGCCGCGTCGCCGCTTC-3′
G25-2	TGATTATCTTTCTAGGG	TTAA	CAATCAATAAGATAAACATACACAGA
G25-3	TGATTATCTTTCTAGGG	TTAA	CACCACATTTAACTTGCTCTTTGATA
G28-1	TGATTATCTTTCTAGGG	TTAA	TAGAGTGCTGAGATTTGGGACATTGC
G29-1	TGATTATCTTTCTAGGG	TTAA	GGCGTTGGTGGCACACAACTTTAAGT
G34-2	TGATTATCTTTCTAGGG	TTAA	TAAGACAATGTATGACTTTGTCCCAT

Six clones were analyzed, and all demonstrate that GAL4-piggyBac mediated integration into a TTAA site. The raw sequence data are provided in Fig. 5.