Table 1.
Effect of NLS and PKA mutations on Maf1 function
| Mutations | Pol III repression* | Glycerol 37°C† | Growth SGal-His‡ | ade2-1 color§ | Location¶ |
|
|---|---|---|---|---|---|---|
| −rap | +rap | |||||
| WT | 28 | ++++ | − | − | N + C | N |
| maf1Δ | 112 | − | ++ | +++ | − | − |
| 6SA | 26 | ++++ | − | − | N > C | N |
| 6SE | 28 | ++++ | − | − | C > N | N |
| 6SAΔCtNLS | 42 | ++++ | (+) | + | N > C | N |
| 6SEΔCtNLS | 91 | +++ | +++ | ++ | C | C > N |
| ΔNtNLS | 35 | ++++ | − | − | C | C > N |
| ΔNtCtNLS | 101 | +++ | +++ | ++ | C | C |
| ΔCtNLS | 64 | +++ | +++ | ++ | C | N |
*Residual transcription after 90 min of rapamycin treatment expressed as a percentage of the untreated WT level (from Fig. 4). Data is representative of multiple experiments that generate an SD of 3–8%. An examination of unstressed transcription for many Maf1 mutants found no significant changes compared with WT or the maf1Δ strain.
†Glycerol phenotype: + represents each 10-fold dilution at which cells grew on SGly-Trp at 37°C.
‡tgm-silencing phenotype: + represents each 10-fold dilution at which cells grew on SGal-Ura-Trp-His at 30°C.
§Antisuppression: −, cream color; +, light pink; ++, pink; +++, red on SGal-Ura-Trp media.
¶Localization: Cytoplasmic (C) and nuclear signal (N) after 60 mins with (+) or without (−) rapamycin treatment. Qualitative changes in distribution are expressed relative to WT.