Fig. 1.

AR protein expression during cell cycling of AS prostate cancer cells. (A) AR protein is expressed strongly in CWR22Rv1, LNCaP, and LAPC-4, is very weakly expressed in human PrSCs, and is not expressed in PC3 and DU145 cells. (B–D) Dual-parameter FACS analyses for AR expression and DNA fluorescence analysis in PC3 (B), LNCaP (C), and CWR22Rv1 (D) prostate cancers cells. Results are presented as linear scale dot plots with DNA fluorescence (i.e., cell cycle stage) on the x axis and AR staining intensity on y axis. All analyzed cells were grown in culture in the presence of 1.0 nM R1881. PC3 expression pattern was used to set up a gate to identify specific (i.e., detectable) vs. nonspecific (i.e., nondetectable) staining for AR. This gate was then applied to all subsequent FACS analyses.