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. 2006 Oct 2;103(41):15085–15090. doi: 10.1073/pnas.0603057103

Fig. 3.

Fig. 3.

AR protein is not expressed in mitotic AS prostate cancer cells. (A) Flow sorting of exponentially growing LNCaP cells positive for phospho-histone H3 expression to select cells in mitosis. (B and C) Immunocytochemical analysis of AR expression in exponentially growing (control) LNCaP and LAPC-4 prostate cancer cells compared with phospho-histone H3+ (i.e., mitotic) sorted cells. Compared with exponentially growing (i.e., nonmitotic) AR+ LNCaP and LAPC-4 control cells (B Left and C Left), AR expression was undetectable in phospho-histone H3+ prostate cancer cells (B Center and Right and C Center and Right). (D) LNCaP cells were treated with MG-132 proteasome inhibitor for 6 h in the presence of R1881, flow sorted for phospho-histone H3 expression, and analyzed for AR expression by immunocytochemistry. Proteasome inhibition resulted in strong AR expression during mitosis.