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AR expression during cell cycling of androgen-insensitive prostate stromal cells. (A) Normal human PrSCs (PrSC cells nos. 1–4, 6, and 12) were exponentially growing or arrested in G0 in the presence of androgen by contact inhibition. Protein lysates were immunoblotted for the expression of AR and the DNA replication licensing factor MCM2. (B) Dual-parameter FACS analysis of proliferating PrSC cell no. 6 shows that AR levels fluctuate from 1× to 2× throughout the cell cycle.
