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A. ceylanicum extracts contain Cry5B-binding glycolipids. Upper-phase glycolipids were extracted from mixed-stage C. elegans (Ce) and adult A. ceylanicum (Ac) and separated by TLC. The separated glycolipids were then subjected to a Cry5B overlay in the presence of 80 mM glucose (Glc) (left lanes) or 80 mM galactose (Gal) (right lanes). Cry5B bound to glycolipids from both nematodes in a galactose-dependent fashion.
