Fig. 1.

TEL-AML1 transgenic zebrafish with ubiquitous and lymphoid-restricted expression. (A) Diagrams of the human TEL-AML1c (TA) cDNA, alone or fused in-frame to EGFP, expressed from the ubiquitous Xenopus elongation factor-1 (XEF) 0.7 kb, the zebrafish β-actin (ZBA) 4.5-kb promoters, or from the lymphoid zebrafish Recombination Activation Gene-2 (ZRAG2) 6.5-kb promoter. (B) Transgenic embryos (T) from the ZBA-EGFP-TA line expressing EGFP-TEL-AML1 at 3 dpf compared with nontransgenic (NT) sibling. Fish were oriented with anterior to the left and dorsal to the top (T) or dorsal to the bottom (NT). (C) Ventral view of a 7-dpf RAG2-EGFP-TA zebrafish with EGFP-TEL-AML1-labeled cells in the bilateral thymus (Th) (arrowheads). (Scale, 1 mm in B and 2 mm in C.)