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. 2006 Oct 2;103(41):15166–15171. doi: 10.1073/pnas.0603349103

Fig. 4.

Fig. 4.

Molecular analysis of TEL-AML1 induced leukemia in transgenic zebrafish. (A) Southern blotting of RT-PCR products from five leukemia samples and positive control. (B) Semiquantitative RT-PCR showing the relative expression of each regulatory gene expressed as a ratio to β-actin to normalize the number of leukemic blasts. First, the expression range (dotted lines) in kidney marrow cells from wild-type and nonleukemic TEL-AML1 transgenic fish was established (C, control; n = 15). The expression levels from each of the five TEL-AML1 leukemic zebrafish are depicted (L, leukemias n = 5). (C) Down-regulation of zebrafish TEL transcripts in TEL-AML1 induced leukemia measured by quantitative one-step real-time RT-PCR. The value is presented as a ratio normalized to β-actin when a ratio of 1 represents the normalized expression ratio in wild-type. (D) Apoptotic signal changes in leukemic fish displayed as the mean (± SD) transcript copy number relative to β-actin. A survival determinant high Bcl2/Bax ratio was 7- to 8-fold higher than control in L2 (∗, P = 0.004), L3 (∗, P = 0.0043), and L5 (∗, P = 0.0063). Data represent three independent experiments done in triplicates.