Fig. 3.

Summary of in situ-verified singing-regulated genes. (A) Table of inferred cellular location, molecular function, and biological process based on ontology definitions of homologous genes in other species. The list is organized according to cellular location (nucleus-to-extracellular space), proportion of vocal nuclei, peak time (0.5–3 h), and temporal patterns (types I–VI) of expression. Sim, similar to, at 60–74% protein identity (Appendix section 3.6.6). (B and C) Pie-chart quantifications of cellular location (B) and molecular function (C). (D) Percentage of the 33 genes regulated by singing in each vocal nucleus. The numbers of genes regulated are in parentheses.