Fig. 2.
Dopamine induces SR2-GFP-receptor internalization in SB1 cells. (A–D) Most 5-HT2A receptors were localized to the plasma membrane of SB1 cells after cycloheximide treatment. Cells were then incubated in various concentrations of dopamine for 10 min. (E and F) Internalization of the receptor is seen at 5 μM dopamine (E) and increased on application of 10 μM dopamine (F). Less than 5 μM dopamine, i.e., 1 μM (B), 3 μM (C), and 4 μM (D) dopamine, did not induce any observable internalization of the receptor. (Scale bar, 50 μm.)