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. 2003 Jul 1;112(1):67–75. doi: 10.1172/JCI16645

Figure 4.

Figure 4

Host AT1a receptor is expressed on tumor-associated macrophages. (a) RT-PCR analysis for AT1a mRNA shows cultured B16-F1 melanoma cells, and implanted tumor tissues express AT1a mRNA. Subcutaneous tissues surrounding tumors expressed AT1a mRNA in WT mice but only slightly in AT1a–/– mice. (b) RT-PCR analysis for β-galactosidase (β-gal) mRNA in AT1a–/– mice shows subcutaneous tissues surrounding tumors express β-galactosidase (equivalent expression site of host AT1a receptor). β-Galactosidase mRNA is little expressed in tumors, indicating the absence of the host AT1a receptor within tumor tissues. (c) Subcutaneous tissues isolated from a remote normal skin and tumor-implanted site were stained with an FITC-conjugated anti–β-galactosidase mAb (representing host AT1a receptor) (FITC–β-gal) and phycoerythrin-conjugated anti-macrophage mAb (PE-macrophage). Panels indicate that macrophages located around tumors (TAMs) express β-galactosidase (host AT1a receptor). Bars indicate 100 μm. T, tumor.