Figure 2.

CD25 expression on naive and memory CD4 subsets. B10.D2 spleen cells enriched for CD4 cells as described in Methods were stained with mAb’s against CD4, CD25, CD62L, and CD44. We found that 12.4% of CD4 cells were CD25⁺ (a). We gated on CD62L⁺CD44^– naive and CD62L^–CD44⁺ memory CD4 cells (b) and analyzed their expression of CD25 (c and d). Note that 33.1% of cells with a memory phenotype express CD25 versus 5.1% of cells with a naive phenotype.