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. 2006 Oct 6;103(42):15529–15534. doi: 10.1073/pnas.0607262103

Fig. 1.

Fig. 1.

Expression of Cav channel subunits by CD4 T cells. Purified total CD4 T cells (CD4+) or naïve T cells (CD62L-high and CD44-low) were left unstimulated (day 0) or were stimulated with plate-bound anti-CD3 plus anti-CD28 Abs for the indicated period. (A and B) Analysis of β4 and β3 subunit mRNA expression by CD4 T cells using real-time quantitative PCR. (C) Western blot analysis of β3 subunit expression in CD4 T cells. (D) Expression of the Cav channel α1 subunits by CD4 T cells at different stages of differentiation. (E) Expression of Cav channel subunits by β4 mutant T cells assessed by using RT-PCR and Western blot. (F) Western blot analysis of the expression of β3 subunit in effector WT or β3 KO T cells. Results in A and D are representative of at least three independent experiments. For B, C, E, and F, two experiments were performed.