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. 2006 Oct 9;103(42):15570–15575. doi: 10.1073/pnas.0604728103

Fig. 1.

Fig. 1.

GB virus C NS5A expression. (A) The predicted genome organization of GBV-C is shown, including the 5′ nontranslated region [containing an internal ribosomal entry site (IRES)], envelope glycoproteins (E1 and E2) nonstructural proteins (NS), and 3′ nontranslated region. The predicted 414-aa-long NS5A protein is encoded by nucleotides 6150–7392 (GenBank accession no. AF121950) with the putative N-terminal membrane anchoring domain shown in yellow. (B) GFP expression was detected by flow cytometry in Jurkat cells alone (black) or stably transfected with VC (red), VC-GFP (blue), or expressing the NS5A from an IFN-S isolate (GenBank accession no. DQ177420 in green) and an IFN-R isolate (DQ177421) in purple. (C) Immunoreactive GBV-C NS5A proteins from both the IFN-S and IFN-R isolates were observed in GFP-positive clones, and NS5A expression was reduced when cells were maintained in doxycycline for 48 h before preparing cell lysates.