Fig. 2.
Expression of GBV-C NS5A in Jurkat cells inhibits HIV-1 replication. (A) Cells containing IFN-R or IFN-S GBV-C NS5A or VC or VC-GFP were infected with HIV-1 (isolate no. 1073) after doxycyline was removed for 4 days. HIV-1 p24 antigen released into culture supernatants was statistically greater in control cells on days 4–6 after HIV-1 infection. ∗, P < 0.03; ∗∗, P < 0.001. Infections were performed in triplicate, and SE for IFN-R NS5A and vector control cells are shown. (B) The HIV-1 infectious titer (TCID50 in PBMC culture) of culture supernatants was determined for cells expressing the VC-GFP or the IFN-S NS5A.