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. 2006 Oct 9;103(42):15570–15575. doi: 10.1073/pnas.0604728103

Fig. 3.

Fig. 3.

Dose relationship between GB virus C NS5A expression and HIV replication in Jurkat cells as well as NS5A expression in infected PBMC cultures. (A and B) Jurkat cells expressing VC-GFP (A) or IFN-S NS5A (B) were maintained in 0, 1, or 5 μg/ml doxycycline. Doxycycline had no significant effect on HIV-1 replication in VC-GFP cells (A), yet in Jurkat cells expressing NS5A, HIV-1 replication increased with increases in doxycycline concentration (decreased NS5A expression; B). HIV-1 replication was significantly lower (>90%) than that observed in control cells when maintained in 5 μg/ml doxycycline. (C) NS5A demonstrates immunoreactive protein in PBMC lysates (200,000 cells) 3 days after GBV-C (G) or mock infection (M) and Jurkat cells with NS5A (5A) or the VC-GFP.