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. 2006 Oct 9;103(42):15687–15692. doi: 10.1073/pnas.0606195103

Fig. 5.

Fig. 5.

MGL expression patterns in response to Met and in different Arabidopsis organs. (A) MGL protein expression in Arabidopsis cells treated or not treated with 2 mM Met. Total soluble proteins (20 μg per lane) were analyzed by Western blot analysis using antibodies against AtMGL. At each time point, the ratio between MGL levels in treated versus control cells was measured by using the ECL Plus Western Blotting detection reagents and a Typhoon 9400 scanner (data are means ± SD from two independent cell cultures). (B) AtMGL mRNA and protein expression levels in Arabidopsis organs. Protein expression was analyzed by Western blotting as described above: cell suspension cultures (C), roots (R), stems (S), rosette leaves (L), flowers (F), siliques (S), dry mature seeds (DS), and seeds imbibed with water for 24 h at 4°C (IS). Steady-state AtMGL (At1g64660) mRNA levels were obtained from the Arabidopsis microarray database Genevestigator (www.genevestigator.ethz.ch). The protein and mRNA levels relative abundances were referred to the expression found in cell suspension cultures. #, no detectable protein; ∗, no data available from the microarray database.