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. 2000 Mar 28;97(7):3276–3281. doi: 10.1073/pnas.97.7.3276

Figure 2.

Figure 2

mog-4 and mog-5 mRNA expression. (A) mog-4 and mog-5 RNAs during development. In each lane, 3.8 μg of poly(A)-enriched RNA was loaded from embryos (E), larvae [first larval stage (L1), early L2 (eL2), late L2 (lL2), and fourth larval stage (L4)] and adults (A). Probes were the full-length cDNAs for mog-4 (pAP5) and mog-5 (pJK615), respectively. Molecular sizes were determined by comparison to Promega RNA markers. CeIF encodes the C. elegans homolog of eukaryotic initiation factor 4A, which is expressed at a constant level throughout development (28). (B) mog-4 and mog-5 RNAs are present in soma and germ line. RNA was derived from wild-type (N2) adults and glp-1(q224) adults raised at restrictive temperature, which have virtually no germ line (29). Each lane was loaded with 2.5 μg of poly(A)-enriched RNA and probed with mog-4, mog-5, and C. elegans actin-1-specific (as loading control) cDNAs. The mog-4 and mog-5 transcripts are abundant in wild-type RNA but much reduced in animals lacking a germ line. For comparison, blots were also probed with mog-1 (A and not shown).