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. 2003 Jul 1;112(1):42–49. doi: 10.1172/JCI17856

Figure 3.

Figure 3

Ischemia mobilizes Lin–Sca-1+ cells. Peripheral blood from a mouse 24 hours after sham operation (a) or 25 minutes of unilateral I/R (b) was labeled for detection of Lin+ and Sca-1+ cells and analyzed on a FACS. Lin–Sca-1+ stem cells were not seen in the circulation of the control animals (a, lower right quadrant); the 1.4% of cells detected is equal to the value seen with the unrelated isotype control antibody and is therefore indistinguishable from background. In contrast, 23.8% of the circulating cells were Lin–Sca-1+ stem cells 24 hours after I/R (b, lower right quadrant).