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. 2003 Jul 1;112(1):136–142. doi: 10.1172/JCI18161

Figure 3.

Figure 3

Detection of RNA transcripts of CSR-related genes. (a) RNA transcripts of the CD19 (as control), AID, germ-line (εGLTs), circular (εCTs), and functional (εFTs) transcripts for IgE, and of the functional transcripts for IgG (γFTs) were detected by RT-PCR after stimulation with sCD40L+rIL-4 for 5 days in control subjects (C) and in HIGM4 patients (P1, P2, P5, P7, P8, P11, P15). (b) CD19 transcripts and εGLTs levels were analyzed using different quantities of cDNA (1:1, 1:10, 1:100, and 1:1,000 dilution) in RT-PCR for selected cases (P1, P5, P7, P15).